Review Questions
- DNA to mRNA to protein
 - DNA to mRNA to tRNA to protein
 - DNA to protein to mRNA to protein
 - mRNA to DNA to mRNA to protein
 
- DNA and protein from B
 - DNA and protein from A
 - DNA from A and protein from B
 - DNA from B and protein from A
 
The AUC and AUA codons in mRNA both specify isoleucine. What feature of the genetic code explains this?
- complementarity
 - degeneracy
 - nonsense codons
 - universality
 
Which of the following molecules does not contain genetic information?
- DNA
 - mRNA
 - protein
 - RNA
 
Which molecule in the central dogma can be compared to a disposable photocopy of a book kept on reserve in the library?
- DNA
 - mRNA
 - protein
 - tRNA
 
Which subunit of the E. coli polymerase confers specificity to transcription?
- α
 - β
 - β′
 - σ
 
Why are the −10 and −35 regions of prokaryotic promoters called consensus sequences?
- They are identical in all bacterial species.
 - They are similar in all bacterial species.
 - They exist in all organisms.
 - They have the same function in all organisms.
 
The sequence that signals the end of transcription is called the ________.
- promoter.=
 - stop codon.=
 - TATA box.=
 - terminator.=
 
- It depends on the gene.
 - No, the rho protein is essential.
 - Transcription termination is not required.
 - Yes, the rho protein is not involved in transcription.
 
- GC box
 - octamer box
 - TATA box
 - -10 and -35 sequences
 
- elongation
 - initiation
 - processing
 - termination
 
- polymerase I
 - polymerase II
 - polymerase III
 - ribonuclease I
 
What transcripts will be most affected by low levels of α-amanitin?
- 18S and 28S rRNAs
 - 5S rRNAs and tRNAs
 - other small nuclear RNAs
 - pre-mRNAs
 
- Eukaryotic transcription does not start at a consensus sequence.
 - Eukaryotic transcription does not require an initiation complex.
 - Eukaryotic transcription and translation do not take place at the same time.
 - Eukaryotic transcription does not require a termination sequence.
 
A poly-A sequence is added at the:
- 5′ end of a transcript in the nucleus
 - 3′-end of a transcript in the nucleus
 - 5′ end of a transcript in the cytoplasm
 - 3′-end of a transcript in the cytoplasm
 
- poly-A tail
 - RNA editing
 - splicing
 - 7-methylguanosine cap
 
- cytoplasm
 - endoplasmic reticulum
 - nucleus
 - nucleolus
 
- cleavage
 - methylation
 - nucleotide modification
 - splicing
 
- DNA sequences to which polymerases bind
 - the processed mRNA
 - translated DNA sequences in a gene
 - untranslated DNA sequences in a gene
 
- adenine
 - leucine
 - methionine
 - thymine
 
In any given species, there are at least how many types of aminoacyl tRNA synthetases?
- 20
 - 40
 - 100
 - 200
 
In prokaryotic cells, ribosomes are found in/on the ________.
- cytoplasm
 - mitochondrion
 - nucleus
 - endoplasmic reticulum
 
The peptide bond synthesis in prokaryotic translation is catalyzed by ________.
- a ribosomal protein
 - a cytoplasmic protein
 - mRNA itself
 - ribosomal RNA
 
What would happen if the 5′-methyl guanosine was not added to an mRNA?
- The transcript would degrade when the mRNA moves out of the nucleus to the cytoplasm.
 - The mRNA molecule would stabilize and start the process of translation within the nucleus of the cell.
 - The mRNA molecule would move out of the nucleus and create more copies of the mRNA molecule.
 - The mRNA molecule would not be able to add the poly-A tail on its strand at the 5′ end.
 
Which of the following is associated with the docking of mRNA on a ribosome in eukaryotic cells?
- Kozak’s rules
 - poly-A sequence
 - Shine-Dalgarno sequence
 - TATA box